7CMJ
Crystal structure of L.donovani Hypoxanthine-guanine phosphoribosyl transferase (HGPRT)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 11.2C |
Synchrotron site | ELETTRA |
Beamline | 11.2C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9784 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 80.800, 80.800, 344.321 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.370 - 2.760 |
R-factor | 0.183 |
Rwork | 0.181 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pzm |
RMSD bond length | 0.008 |
RMSD bond angle | 0.933 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.373 | 2.860 |
High resolution limit [Å] | 2.760 | 2.760 |
Rmerge | 0.042 | 0.292 |
Number of reflections | 17049 | 1731 |
<I/σ(I)> | 10.15 | |
Completeness [%] | 93.7 | 98.1 |
Redundancy | 1.9 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | 0.1M Sodium cacodylate, 0.2 M Ammonium sulfate, 20% PEG 4000 |