7CMG
The Structure of the periplasmic domain of PorM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-05 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 87.140, 98.280, 209.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 80.470 - 3.700 |
R-factor | 0.2177 |
Rwork | 0.213 |
R-free | 0.25820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ey5 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.450 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 88.990 | 4.050 |
High resolution limit [Å] | 3.700 | 3.700 |
Rpim | 0.103 | 0.180 |
Number of reflections | 19679 | 4681 |
<I/σ(I)> | 6.4 | 4.2 |
Completeness [%] | 99.0 | |
Redundancy | 3.8 | |
CC(1/2) | 0.974 | 0.884 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 14.6% (w/v) ethanol, 0.1 M Tris-HCl pH8.5, 150 mM NaCl, and 1mM taurine |