7CMA
Structure of A151R from African swine fever virus Georgia
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-07-27 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 0.9998 |
Spacegroup name | P 32 |
Unit cell lengths | 49.869, 49.869, 128.081 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.490 - 2.010 |
R-factor | 0.2497 |
Rwork | 0.249 |
R-free | 0.25850 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 25.000 | 25.000 | 2.080 |
High resolution limit [Å] | 2.010 | 4.321 | 2.010 |
Rmerge | 0.042 | 0.032 | 0.415 |
Rmeas | 0.048 | 0.037 | 0.482 |
Rpim | 0.023 | 0.017 | 0.243 |
Total number of observations | 97465 | ||
Number of reflections | 23627 | 2289 | 2345 |
<I/σ(I)> | 23.4 | ||
Completeness [%] | 99.0 | 94.9 | 98.6 |
Redundancy | 4.1 | 4.3 | 3.7 |
CC(1/2) | 0.998 | 0.866 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | polyethylene glycol 20000, 0.1M MES pH 6.5 |