7CM1
Neuraminidase from the Wuhan Asiatic toad influenza virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-01-06 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 119.966, 143.148, 120.097 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.350 - 2.140 |
R-factor | 0.2234 |
Rwork | 0.220 |
R-free | 0.27960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1b9s |
RMSD bond length | 0.007 |
RMSD bond angle | 0.994 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (dev_3940) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.220 |
High resolution limit [Å] | 2.140 | 2.140 |
Number of reflections | 55993 | 5151 |
<I/σ(I)> | 7.2 | |
Completeness [%] | 97.6 | |
Redundancy | 3.5 | |
CC(1/2) | 0.995 | 0.760 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 8 mg/mL in 20 mM Tris, 50 mM NaCl pH 8.0 |