7CLR
CryoEM structure of S.typhimurium flagellar LP ring
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | JEOL CRYO ARM 200 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 1.4 |
| Energy Filter | In-column Omega Filter |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.5 |
| Number of Particle | 64418 |
| Software | PHENIX,Coot,RELION,Gctf,UCSF Chimera |
| Symmetry | C26 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
