7CLK
Mycobacterium tuberculosis enolase in complex with alternate 2-phosphoglycerate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2019-03-15 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54178 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 139.804, 139.804, 89.645 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.990 - 2.150 |
R-factor | 0.1448 |
Rwork | 0.142 |
R-free | 0.19080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5j04 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.503 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.080 | 0.545 |
Number of reflections | 29616 | 2875 |
<I/σ(I)> | 13.39 | |
Completeness [%] | 97.9 | 96.4 |
Redundancy | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 20% PEG 3350, 0.2M ammonium acetate, Bis-Tris pH 6.5 |