7CLG
Crystal structure of the ATP-dependent restriction endonuclease SauUSI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-11 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97954 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 86.583, 175.679, 89.379 |
Unit cell angles | 90.00, 115.68, 90.00 |
Refinement procedure
Resolution | 56.790 - 3.100 |
R-factor | 0.2472 |
Rwork | 0.245 |
R-free | 0.29670 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.752 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | SOLVE |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 87.800 | 3.220 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.110 | 1.200 |
Number of reflections | 85717 | 4572 |
<I/σ(I)> | 13.1 | |
Completeness [%] | 99.9 | |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M sodium citrate, 11% to 14% PEG 4000' 0.25 M to 0.4 M ammonium sulfate |