7CLE
Non-Specific Class-c acidphosphatase from Sphingobium sp. RSMS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
Synchrotron site | RRCAT INDUS-2 |
Beamline | PX-BL21 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2018-10-05 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.979500 |
Spacegroup name | I 41 3 2 |
Unit cell lengths | 170.622, 170.622, 170.622 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.656 - 2.342 |
R-factor | 0.1931 |
Rwork | 0.192 |
R-free | 0.22310 |
Structure solution method | AB INITIO PHASING |
RMSD bond length | 0.003 |
RMSD bond angle | 0.617 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MR-Rosetta |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.600 | 2.420 |
High resolution limit [Å] | 2.340 | 2.340 |
Number of reflections | 18061 | 1632 |
<I/σ(I)> | 15.7 | |
Completeness [%] | 99.4 | |
Redundancy | 28.9 | |
CC(1/2) | 0.998 | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 5.5 | 294 | 1M Ammonium sulphate, 0.1 M Bis-tris pH 5.5, 1% PEG 3350 |