7CKV

Crystal structure of Cyanobacteriochrome GAF domain in Pr state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AichiSR BEAMLINE BL2S1
Synchrotron site	AichiSR
Beamline	BL2S1
Temperature [K]	95
Detector technology	CCD
Collection date	2020-01-29
Detector	ADSC QUANTUM 270
Wavelength(s)	1.12
Spacegroup name	P 1 21 1
Unit cell lengths	35.730, 101.334, 41.810
Unit cell angles	90.00, 91.79, 90.00

Refinement procedure

Resolution	38.663 - 1.630
Rwork	0.158
R-free	0.19910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3vv4
RMSD bond length	0.011
RMSD bond angle	1.718
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.790	1.660
High resolution limit [Å]	1.630	1.630
Rmerge	0.046	0.483
Rmeas	0.054	0.562
Rpim	0.027	0.285
Number of reflections	36876	1810
<I/σ(I)>	13.3	2.2
Completeness [%]	99.6	99.9
Redundancy	3.9	3.9
CC(1/2)	0.996	0.837

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	293	27% PEG 4000, 0.2M MgCl2, 0.1M Tris-HCl