7CKV
Crystal structure of Cyanobacteriochrome GAF domain in Pr state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AichiSR BEAMLINE BL2S1 |
Synchrotron site | AichiSR |
Beamline | BL2S1 |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2020-01-29 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.12 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 35.730, 101.334, 41.810 |
Unit cell angles | 90.00, 91.79, 90.00 |
Refinement procedure
Resolution | 38.663 - 1.630 |
Rwork | 0.158 |
R-free | 0.19910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vv4 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.718 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.790 | 1.660 |
High resolution limit [Å] | 1.630 | 1.630 |
Rmerge | 0.046 | 0.483 |
Rmeas | 0.054 | 0.562 |
Rpim | 0.027 | 0.285 |
Number of reflections | 36876 | 1810 |
<I/σ(I)> | 13.3 | 2.2 |
Completeness [%] | 99.6 | 99.9 |
Redundancy | 3.9 | 3.9 |
CC(1/2) | 0.996 | 0.837 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 27% PEG 4000, 0.2M MgCl2, 0.1M Tris-HCl |