7CKP
Mycobacterium tuberculosis Enolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9775 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 141.062, 141.062, 95.536 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.920 - 2.900 |
| R-factor | 0.24706 |
| Rwork | 0.243 |
| R-free | 0.29150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6KKC |
| RMSD bond length | 0.011 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 10414 | 388 |
| <I/σ(I)> | 23.69 | 2.5 |
| Completeness [%] | 94.7 | |
| Redundancy | 21.6 | |
| CC(1/2) | 0.981 | 0.826 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 20% PEG 3350, 0.2 M ammonium acetate, Bis-Tris pH 6.5 |
