7CKP
Mycobacterium tuberculosis Enolase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9775 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 141.062, 141.062, 95.536 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.920 - 2.900 |
R-factor | 0.24706 |
Rwork | 0.243 |
R-free | 0.29150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6KKC |
RMSD bond length | 0.011 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.950 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 10414 | 388 |
<I/σ(I)> | 23.69 | 2.5 |
Completeness [%] | 94.7 | |
Redundancy | 21.6 | |
CC(1/2) | 0.981 | 0.826 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 20% PEG 3350, 0.2 M ammonium acetate, Bis-Tris pH 6.5 |