7CKA
The structure of Glycine max (Soybean) Heme oxygenase 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 98 |
Detector technology | PIXEL |
Collection date | 2019-04-22 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.90000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.748, 45.649, 72.767 |
Unit cell angles | 90.00, 101.16, 90.00 |
Refinement procedure
Resolution | 37.050 - 1.060 |
Rwork | 0.159 |
R-free | 0.17290 |
Structure solution method | SAD |
Data reduction software | XDS (Mar 15, 2019) |
Data scaling software | XDS (Mar 15, 2019) |
Phasing software | PHASER (1.13) |
Refinement software | SHELX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.050 | 1.130 |
High resolution limit [Å] | 1.060 | 1.060 |
Rmerge | 0.051 | 0.456 |
Number of reflections | 107574 | 17057 |
<I/σ(I)> | 9.78 | 1.93 |
Completeness [%] | 98.3 | 97.1 |
Redundancy | 2.81 | 2.64 |
CC(1/2) | 0.997 | 0.752 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.1 | 277.15 | 60 mM Citric acid, 40 mM BIS-TRIS propane / pH 4.1, 16% w/v Polyethylene glycol 3350, 3.2%Dimethyl sulfoxide |