7CKA
The structure of Glycine max (Soybean) Heme oxygenase 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 98 |
| Detector technology | PIXEL |
| Collection date | 2019-04-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.748, 45.649, 72.767 |
| Unit cell angles | 90.00, 101.16, 90.00 |
Refinement procedure
| Resolution | 37.050 - 1.060 |
| Rwork | 0.159 |
| R-free | 0.17290 |
| Structure solution method | SAD |
| Data reduction software | XDS (Mar 15, 2019) |
| Data scaling software | XDS (Mar 15, 2019) |
| Phasing software | PHASER (1.13) |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.050 | 1.130 |
| High resolution limit [Å] | 1.060 | 1.060 |
| Rmerge | 0.051 | 0.456 |
| Number of reflections | 107574 | 17057 |
| <I/σ(I)> | 9.78 | 1.93 |
| Completeness [%] | 98.3 | 97.1 |
| Redundancy | 2.81 | 2.64 |
| CC(1/2) | 0.997 | 0.752 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.1 | 277.15 | 60 mM Citric acid, 40 mM BIS-TRIS propane / pH 4.1, 16% w/v Polyethylene glycol 3350, 3.2%Dimethyl sulfoxide |
