7CK3

Crystal structure of Arabidopsis CESA3 catalytic domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-08-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	49.140, 58.430, 321.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.520 - 2.900
R-factor	0.245
Rwork	0.244
R-free	0.26780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ck1
RMSD bond length	0.013
RMSD bond angle	1.395
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.980
High resolution limit [Å]	2.900	2.900
Number of reflections	21506	1457
<I/σ(I)>	6.55	0.95
Completeness [%]	100.0	99.9
Redundancy	41.19
CC(1/2)	0.990	0.420

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		291	PEG3350