7CK0

Room temperature structure of glucose isomerase delivered in lard by serial millisecond crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	298.15
Detector technology	PIXEL
Collection date	2019-04-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9794
Spacegroup name	I 2 2 2
Unit cell lengths	94.190, 99.920, 103.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.800 - 1.800
R-factor	0.1581
Rwork	0.158
R-free	0.17660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zyd
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.460	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	45422	4492
<I/σ(I)>	5.72	2.81
Completeness [%]	100.0	100
Redundancy	565.1	387.8
CC(1/2)	0.977	0.548

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	7	293.5	Tris-HCl, Ammonium sulfate, Magnesium sulfate