7CJZ

Room temperature structure of lysozyme delivered in lard by serial millisecond crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	298.15
Detector technology	PIXEL
Collection date	2019-04-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9794
Spacegroup name	P 43 21 2
Unit cell lengths	79.450, 79.450, 38.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.180 - 1.750
R-factor	0.1662
Rwork	0.165
R-free	0.18650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ig6
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.000	1.810
High resolution limit [Å]	1.750	1.750
Number of reflections	12954	1256
<I/σ(I)>	9.84	3.6
Completeness [%]	100.0	100
Redundancy	385.2	266.8
CC(1/2)	0.993	0.805

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	4.5	293.5	sodium acetate, PEG 8000, NaCl