7CJZ
Room temperature structure of lysozyme delivered in lard by serial millisecond crystallography
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 11C |
Synchrotron site | PAL/PLS |
Beamline | 11C |
Temperature [K] | 298.15 |
Detector technology | PIXEL |
Collection date | 2019-04-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.450, 79.450, 38.470 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.180 - 1.750 |
R-factor | 0.1662 |
Rwork | 0.165 |
R-free | 0.18650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ig6 |
Data reduction software | CrystFEL |
Data scaling software | CrystFEL |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Number of reflections | 12954 | 1256 |
<I/σ(I)> | 9.84 | 3.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 385.2 | 266.8 |
CC(1/2) | 0.993 | 0.805 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 4.5 | 293.5 | sodium acetate, PEG 8000, NaCl |