7CJS
structure of aquaporin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.11218 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.568, 92.453, 166.126 |
| Unit cell angles | 90.00, 102.14, 90.00 |
Refinement procedure
| Resolution | 19.838 - 1.800 |
| R-factor | 0.2461 |
| Rwork | 0.245 |
| R-free | 0.27320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ne2 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.838 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 237514 | 37334 |
| <I/σ(I)> | 5.73 | |
| Completeness [%] | 96.9 | |
| Redundancy | 3.2 | |
| CC(1/2) | 0.996 | 0.784 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | PEG 400, Gly-NaOH, octyl-glucoside, and cholesteryl hemisuccinate |
