7CJJ

Photosystem II structure in the S2 state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SACLA BEAMLINE BL3
Synchrotron site	SACLA
Beamline	BL3
Temperature [K]	293
Detector technology	CCD
Collection date	2017-04-17
Detector	MPCCD
Wavelength(s)	1.77
Spacegroup name	P 21 21 21
Unit cell lengths	125.750, 231.630, 288.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.902 - 2.400
R-factor	0.1703
Rwork	0.168
R-free	0.21840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	dark structure
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	329844	32695
<I/σ(I)>	37
Completeness [%]	100.0
Redundancy	235
CC(1/2)	0.997	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293	PEG, magnesium sulfate