7CJI

Photosystem II structure in the S1 state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SACLA BEAMLINE BL3
Synchrotron site	SACLA
Beamline	BL3
Temperature [K]	293
Detector technology	CCD
Collection date	2017-04-18
Detector	MPCCD
Wavelength(s)	1.77
Spacegroup name	P 21 21 21
Unit cell lengths	126.020, 231.700, 288.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.932 - 2.350
R-factor	0.1628
Rwork	0.160
R-free	0.20760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ws5
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.430
High resolution limit [Å]	2.350	2.350
Number of reflections	351162	34826
<I/σ(I)>	56.8	2
Completeness [%]	100.0	100
Redundancy	494	339
CC(1/2)	0.999	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293	PEG, magnesium sulfate