7CIR
Peptide phosphorylation modification of MHC class I molecules
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-18 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.725, 82.238, 108.441 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.940 - 1.810 |
R-factor | 0.1781 |
Rwork | 0.176 |
R-free | 0.20770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bst |
RMSD bond length | 0.006 |
RMSD bond angle | 0.844 |
Phasing software | CNS |
Refinement software | REFMAC (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.111 | 0.517 |
Number of reflections | 303959 | 303959 |
<I/σ(I)> | 3.8 | |
Completeness [%] | 98.5 | |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.1M TRIS-HCl (PH7.9) 17.5%(w/v)PEG8000 |