7CHX
acylphosphatase from Staphylococcus aureus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-25 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97940 |
Spacegroup name | P 43 |
Unit cell lengths | 77.124, 77.124, 35.319 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.720 - 1.490 |
R-factor | 0.2031 |
Rwork | 0.201 |
R-free | 0.23990 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.491 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.520 |
High resolution limit [Å] | 1.490 | 1.490 |
Number of reflections | 32259 | 1417 |
<I/σ(I)> | 26.94 | |
Completeness [%] | 94.1 | |
Redundancy | 3.5 | |
CC(1/2) | 0.984 | 0.901 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM Tris, pH 8.5, 30% (w/v) PEG4000, 200 mM Sodium acetate |