7CHS
Crystal structure of SARS-CoV-2 antibody P22A-1D1 with RBD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-05-17 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9769 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 193.337, 86.598, 57.160 |
Unit cell angles | 90.00, 99.25, 90.00 |
Refinement procedure
Resolution | 47.706 - 2.401 |
R-factor | 0.1925 |
Rwork | 0.191 |
R-free | 0.22440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6m0j |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.460 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.114 | 0.983 |
Number of reflections | 36392 | 3573 |
<I/σ(I)> | 13.1 | |
Completeness [%] | 100.0 | |
Redundancy | 6.7 | |
CC(1/2) | 0.989 | 0.655 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1M potassium chloride, 0.1M NaHEPES, pH 7.0, 15% PEG 5000MME |