7CHP

Crystal structure of SARS-CoV-2 antibody P5A-3C8 with RBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9796
Spacegroup name	P 21 21 2
Unit cell lengths	112.544, 171.566, 54.873
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.984 - 2.357
R-factor	0.1947
Rwork	0.193
R-free	0.21980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m0j
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.220	2.480
High resolution limit [Å]	2.357	2.357
Number of reflections	43632	4424
<I/σ(I)>	13
Completeness [%]	97.3
Redundancy	13.2
CC(1/2)	0.997	0.808

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2M Lithium sulfate monohydrate, 0.1M HEPES, pH 7.5, 25% w/v PEG 3350