7CHL

Crystal structure of hybrid Arabinose isomerase AI-10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-23
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97933
Spacegroup name	P 1 21 1
Unit cell lengths	152.086, 164.297, 151.973
Unit cell angles	90.00, 119.92, 90.00

Refinement procedure

Resolution	30.540 - 3.400
R-factor	0.2108
Rwork	0.208
R-free	0.26420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r1o
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.460
High resolution limit [Å]	3.400	9.210	3.400
Rmerge	0.119	0.038	0.425
Rmeas	0.129	0.042	0.460
Rpim	0.050	0.018	0.173
Total number of observations	544079
Number of reflections	84659	4281	4086
<I/σ(I)>	8.7
Completeness [%]	95.6	93.8	92
Redundancy	6.4	5.5	6.9
CC(1/2)		0.996	0.928

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		310	16% PEG 3350, 0.1M Tris pH 8.5, 2% Tacismate pH 8.0