7CHF

Crystal structure of the SARS-CoV-2 RBD in complex with BD-604 Fab and BD-368-2 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-06-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	99.846, 116.223, 115.766
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.870 - 2.674
R-factor	0.2221
Rwork	0.220
R-free	0.26980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6w41
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.15.2_3472)
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.720
High resolution limit [Å]	2.670	2.670
Number of reflections	37228	2714
<I/σ(I)>	16.2
Completeness [%]	96.0
Redundancy	11.3
CC(1/2)	0.990	0.136

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M ammonium sulfate and 12% (w/v) polyethylene glycol 8000