7CHE

Crystal structure of the SARS-CoV-2 RBD in complex with BD-236 Fab and BD-368-2 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-06-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	100.016, 114.296, 116.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.619 - 3.416
R-factor	0.2262
Rwork	0.225
R-free	0.23880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6w41
RMSD bond length	0.028
RMSD bond angle	2.951
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.11.1_2575)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.510
High resolution limit [Å]	3.416	3.450
Number of reflections	17742	1036
<I/σ(I)>	11.55
Completeness [%]	94.5
Redundancy	12.7
CC(1/2)	0.980	0.692

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M sodium acetate pH 4.0 and 10% (w/v) Polyethylene glycol monomethyl ether 2,000