7CHD
AtaT complexed with acetyl-methionyl-tRNAfMet
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-12 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 269.830, 68.280, 136.180 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.214 - 3.804 |
R-factor | 0.2892 |
Rwork | 0.286 |
R-free | 0.34090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6gtp |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.214 | 48.214 | 3.900 |
High resolution limit [Å] | 3.800 | 17.010 | 3.800 |
Rmerge | 0.441 | 0.083 | 3.416 |
Rmeas | 0.449 | 0.086 | 3.483 |
Total number of observations | 668160 | ||
Number of reflections | 25540 | 323 | 1813 |
<I/σ(I)> | 8.36 | 31.78 | 1.25 |
Completeness [%] | 99.8 | 94.4 | 99 |
Redundancy | 26.161 | 18.254 | 26.185 |
CC(1/2) | 0.999 | 1.000 | 0.468 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Hepes 6.8, 0.2M Sodium formate, 10% PEG3350, 0.5% Jeffemine |