7CHB
Crystal structure of the SARS-CoV-2 RBD in complex with BD-236 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2020-04-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 195.614, 86.788, 56.415 |
Unit cell angles | 90.00, 100.13, 90.00 |
Refinement procedure
Resolution | 48.140 - 2.400 |
R-factor | 0.194 |
Rwork | 0.192 |
R-free | 0.22560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6w41 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.18_3845) |
Refinement software | PHENIX (1.18_3845) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 36363 | 3591 |
<I/σ(I)> | 15.2 | |
Completeness [%] | 99.6 | |
Redundancy | 6.8 | |
CC(1/2) | 0.976 | 0.798 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Sodium Citrate, pH 5.0, 8% (w/v) PEG 8000 |