7CGZ
glucose dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.977 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 160.622, 44.640, 99.885 |
| Unit cell angles | 90.00, 100.10, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.940 |
| Rwork | 0.203 |
| R-free | 0.23470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7cdy |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.718 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rpim | 0.040 | 0.223 |
| Number of reflections | 50863 | 2210 |
| <I/σ(I)> | 25.87 | 2.28 |
| Completeness [%] | 97.8 | |
| Redundancy | 6.4 | |
| CC(1/2) | 0.914 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | PEG4000 |
