7CGV

Full consensus L-threonine 3-dehydrogenase, FcTDH-IIYM (NAD+ bound form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-11
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	61.575, 77.513, 76.834
Unit cell angles	71.01, 75.13, 74.88

Refinement procedure

Resolution	36.410 - 2.380
R-factor	0.2145
Rwork	0.212
R-free	0.26260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wmx
RMSD bond length	0.010
RMSD bond angle	1.176
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.410	2.470
High resolution limit [Å]	2.380	2.380
Number of reflections	48940	4236
<I/σ(I)>	11.4
Completeness [%]	96.2
Redundancy	3.4
CC(1/2)	0.985	0.985

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	2.5mM NAD+, 12% PEG 3350, 0.1M sodium acetate trihydrate (pH 7.0)