7CGU
Crystal Structure of AbHAR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9781 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 68.023, 82.999, 131.148 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.010 - 2.400 |
| R-factor | 0.1966 |
| Rwork | 0.194 |
| R-free | 0.24170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yqd |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.388 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.18.1_3865) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.115 | 0.680 |
| Rpim | 0.037 | 0.267 |
| Number of reflections | 14870 | 766 |
| <I/σ(I)> | 18.4 | 1.89 |
| Completeness [%] | 99.9 | |
| Redundancy | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG 3350, O.2M ammonium sulfate |
