7CGT
RAMEB COMPLEX OF CYCLODEXTRIN GLYCOSYLTRANSFERASE MUTANT
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1993-08 |
| Detector | SIEMENS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 95.000, 104.800, 113.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.000 |
| R-factor | 0.165 |
| Rwork | 0.165 |
| R-free | 0.25700 |
| Structure solution method | X-PLOR WITH WILD-TYPE MODEL |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.700 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.600 | |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 18086 | |
| Completeness [%] | 82.5 | 58 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.7 * | Hofmann, B.E., (1989) J.Mol.Biol., 209, 793. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein solution | 50 (%(v/v)) | |
| 2 | 1 | drop | CGTase | 7-15 (mg/ml) | |
| 3 | 1 | drop | Tris-HCl | 50 (mM) | |
| 4 | 1 | drop | PEG1500 | 0.5 (%) | |
| 5 | 1 | reservoir | ammonium sulphate | 0.9-1.0 (M) | |
| 6 | 1 | reservoir | CGTase | 7-15 (mg/ml) | |
| 7 | 1 | reservoir | Tris-HCl | 50 (mM) | |
| 8 | 1 | reservoir | PEG1500 | 0.5 (%) |
