7CGR
Crystal structure of Azospirillum brasilense L-arabinose 1-dehydrogenase E147A mutant (NADP and glycerol bound form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.857, 90.520, 134.693 |
| Unit cell angles | 90.00, 101.93, 90.00 |
Refinement procedure
| Resolution | 48.934 - 2.093 |
| R-factor | 0.1871 |
| Rwork | 0.185 |
| R-free | 0.22210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6jnk |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.171 | 49.171 | 2.220 |
| High resolution limit [Å] | 2.090 | 6.230 | 2.090 |
| Rmerge | 0.148 | 0.047 | 0.869 |
| Rmeas | 0.176 | 0.055 | 1.040 |
| Total number of observations | 559968 | ||
| Number of reflections | 82746 | 6155 | 26172 |
| <I/σ(I)> | 7.09 | 22.56 | 1.52 |
| Completeness [%] | 99.6 | 99.5 | 99.1 |
| Redundancy | 3.448 | 3.502 | 3.281 |
| CC(1/2) | 0.993 | 0.997 | 0.654 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2 M Lithium sulfate, 0.1 M Tris-HCl pH 8.5, 25% PEG 3350 |
