7CGQ
Crystal structure of Azospirillum brasilense L-arabinose 1-dehydrogenase E147A mutant (NADP and L-arabinose bound form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.078, 92.096, 135.133 |
Unit cell angles | 90.00, 102.25, 90.00 |
Refinement procedure
Resolution | 49.385 - 2.208 |
R-factor | 0.1871 |
Rwork | 0.185 |
R-free | 0.22210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6jnk |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.208 | 2.210 |
Rmerge | 0.085 | 0.740 |
Rmeas | 0.084 | 0.798 |
Number of reflections | 82746 | 21839 |
<I/σ(I)> | 6.45 | 1 |
Completeness [%] | 95.7 | |
Redundancy | 1.8 | |
CC(1/2) | 0.996 | 0.664 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.2M Ammonium citrate tribasic pH 7.0, 18.5% PEG 3350 |