7CGM
Crystal Structure of PUF-8 in Complex with PBE-RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-24 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.977 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 89.203, 89.203, 117.045 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.760 - 2.600 |
R-factor | 0.1881 |
Rwork | 0.185 |
R-free | 0.23540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3q0q |
RMSD bond length | 0.009 |
RMSD bond angle | 1.029 |
Data reduction software | XDS |
Data scaling software | HKL-2000 (V716.1) |
Phasing software | PHASER |
Refinement software | PHENIX (1.10-2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 2.640 |
High resolution limit [Å] | 2.592 | 7.050 | 2.600 |
Rmerge | 0.140 | 0.048 | 0.699 |
Rmeas | 0.146 | 0.050 | 0.731 |
Rpim | 0.043 | 0.015 | 0.214 |
Number of reflections | 15274 | 872 | 747 |
<I/σ(I)> | 5.6 | ||
Completeness [%] | 100.0 | 99.8 | 100 |
Redundancy | 11.5 | 10.1 | 11.4 |
CC(1/2) | 0.999 | 0.964 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 1M LiCl, 0.1M Citric acid (pH 5.0) and 15% PEG6000 |