7CGL
Crystal Structure of PUF-8 in Complex with PBE-RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 89.869, 89.869, 118.015 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.037 - 2.500 |
| R-factor | 0.1983 |
| Rwork | 0.195 |
| R-free | 0.25750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q0q |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.986 |
| Data reduction software | XDS |
| Data scaling software | HKL-2000 (V716.1) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10-2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 6.780 | 2.500 |
| Rmerge | 0.129 | 0.052 | 0.528 |
| Rmeas | 0.135 | 0.055 | 0.551 |
| Rpim | 0.038 | 0.016 | 0.153 |
| Total number of observations | 177494 | ||
| Number of reflections | 16585 | 888 | 813 |
| <I/σ(I)> | 4.3 | ||
| Completeness [%] | 95.5 | 89.2 | 97.6 |
| Redundancy | 10.7 | 9.9 | 10.7 |
| CC(1/2) | 0.998 | 0.929 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 1M LiCl, 0.1M Citric acid (pH 5.0) and 15% PEG6000 |
