7CGJ
Crystal Structure of PUF-8 in Complex with PBE-RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-24 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.977 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 89.355, 89.355, 117.898 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.846 - 2.546 |
R-factor | 0.1933 |
Rwork | 0.191 |
R-free | 0.24160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3q0q |
RMSD bond length | 0.009 |
RMSD bond angle | 1.102 |
Data reduction software | XDS |
Data scaling software | HKL-2000 (V716.1) |
Phasing software | PHASER |
Refinement software | PHENIX (1.10-2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 2.590 |
High resolution limit [Å] | 2.546 | 6.910 | 2.550 |
Rmerge | 0.162 | 0.042 | 0.766 |
Rmeas | 0.170 | 0.044 | 0.804 |
Rpim | 0.051 | 0.014 | 0.243 |
Number of reflections | 16283 | 931 | 784 |
<I/σ(I)> | 4.2 | ||
Completeness [%] | 100.0 | 99.8 | 100 |
Redundancy | 10.8 | 9.6 | 10.5 |
CC(1/2) | 0.999 | 0.936 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 1M LiCl, 0.1M Citric acid (pH 5.0) and 15% PEG6000 |