7CG5
Structure of the sensor domain (long construct) of the anti-sigma factor RsgI4 in Pseudobacteroides cellulosolvens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-25 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.97849 |
Spacegroup name | H 3 2 |
Unit cell lengths | 71.230, 71.230, 127.370 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.610 - 2.850 |
R-factor | 0.2449 |
Rwork | 0.241 |
R-free | 0.28590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7cg1 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.920 |
Data reduction software | XDS (20191211) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.310 | 2.920 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmeas | 0.174 | 0.672 |
Number of reflections | 5638 | 401 |
<I/σ(I)> | 9.12 | 2.58 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.1 | 10.2 |
CC(1/2) | 0.995 | 0.971 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 291 | 0.1 M phosphate-citrate (pH4.2), 0.2 M lithium sulfate and 19% PEG1000 |