7CFX

NAD-soaked Holo-methanol dehydrogenase (MDH) from Methylococcus capsulatus (Bath)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-06-09
Detector	RAYONIX MX300HE
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	127.901, 211.028, 221.723
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.500
Rwork	0.180
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tqo
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.400
High resolution limit [Å]	2.300	2.300
Rpim		0.340
Number of reflections	195256	26426
<I/σ(I)>	13.5
Completeness [%]	99.2	99
Redundancy	6.3
CC(1/2)	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		291	Hepes, PEG600