7CFH
Structure of the CBS domain of the bacterial CNNM/CorC family Mg2+ transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-28 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.520, 61.310, 81.830 |
| Unit cell angles | 90.00, 92.41, 90.00 |
Refinement procedure
| Resolution | 42.560 - 2.000 |
| R-factor | 0.2062 |
| Rwork | 0.204 |
| R-free | 0.23960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | homology model |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.177 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.500 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 30078 | 1987 |
| <I/σ(I)> | 9.28 | |
| Completeness [%] | 100.0 | |
| Redundancy | 8.6 | |
| CC(1/2) | 0.995 | 0.718 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 291 | 0.4 M ammonium thiocyanate, 0.1 M sodium acetate pH 4.5, 15% PEG 4000 |
