7CFF
Structure of the thermostabilized transmembrane domain of the bacterial CNNM/CorC family Mg2+ transporter in complex with Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-02 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 57.490, 83.320, 98.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.270 - 2.000 |
R-factor | 0.2171 |
Rwork | 0.216 |
R-free | 0.23240 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.974 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.300 | 2.120 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 15683 | 2447 |
<I/σ(I)> | 6.99 | |
Completeness [%] | 95.8 | |
Redundancy | 4.7 | |
CC(1/2) | 0.983 | 0.718 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 4 | 291 | 10 mM ZnCl2, 100 mM sodium acetate (pH 4.0), 40% polyethylene glycol 200 |