7CFD
Drosophila melanogaster Krimper eTud2-AubR15me2 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-05-23 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 97.118, 101.365, 191.276 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.992 - 2.704 |
R-factor | 0.2453 |
Rwork | 0.243 |
R-free | 0.29040 |
Structure solution method | SAD |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.114 | 0.511 |
Rmeas | 0.127 | 0.588 |
Rpim | 0.055 | 0.284 |
Number of reflections | 50060 | 3860 |
<I/σ(I)> | 12.5 | 1.52 |
Completeness [%] | 95.5 | 75.1 |
Redundancy | 5.1 | 3.7 |
CC(1/2) | 0.897 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 16% PEG3350 and 0.1 M Ammonium acetate |