7CFC
Drosophila melanogaster Krimper eTud1-Ago3 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-13 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 31 |
| Unit cell lengths | 88.464, 88.464, 181.704 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.513 - 2.400 |
| R-factor | 0.2354 |
| Rwork | 0.234 |
| R-free | 0.25890 |
| Structure solution method | SAD |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.094 | 0.891 |
| Rmeas | 0.107 | |
| Rpim | 0.050 | 0.467 |
| Number of reflections | 61822 | 6092 |
| <I/σ(I)> | 9 | 1.25 |
| Completeness [%] | 99.3 | 98.6 |
| Redundancy | 4.3 | 4.3 |
| CC(1/2) | 0.992 | 0.613 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M HEPES pH 7.5, 1.5M lithium sulfate |
