7CFA
Crystal structure of the restriction DNA glycosylase R.CcoLI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2017-11-17 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 66.493, 89.596, 100.658 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.928 - 2.355 |
R-factor | 0.2306 |
Rwork | 0.229 |
R-free | 0.25920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5iff |
RMSD bond length | 0.002 |
RMSD bond angle | 0.384 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.17) |
Phasing software | PHASER |
Refinement software | PHENIX (1.12) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.170 | 47.170 | 2.440 |
High resolution limit [Å] | 2.350 | 8.810 | 2.350 |
Rmerge | 0.050 | 0.023 | 0.865 |
Rmeas | 0.054 | 0.025 | 0.940 |
Rpim | 0.020 | 0.010 | 0.364 |
Total number of observations | 183674 | 3304 | 17258 |
Number of reflections | 25594 | 560 | 2631 |
<I/σ(I)> | 23.1 | 69.1 | 2.1 |
Completeness [%] | 99.9 | 98.5 | 99.7 |
Redundancy | 7.2 | 5.9 | 6.6 |
CC(1/2) | 0.999 | 0.999 | 0.705 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.1 M MES pH 6.0 and 8% PEG6000 |