7CEM
Crystal Structure of YbeA CP74 W7F
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-10-02 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 128.784, 128.784, 115.964 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.993 - 2.425 |
R-factor | 0.2228 |
Rwork | 0.217 |
R-free | 0.27310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5zyo |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.16-3549) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.520 |
High resolution limit [Å] | 2.425 | 5.230 | 2.430 |
Rmerge | 0.142 | 0.056 | 0.836 |
Rmeas | 0.148 | 0.059 | 0.878 |
Rpim | 0.040 | 0.016 | 0.264 |
Number of reflections | 18788 | 2013 | 1855 |
<I/σ(I)> | 8.6 | ||
Completeness [%] | 100.0 | 100 | 99.8 |
Redundancy | 13.6 | 12.8 | 11 |
CC(1/2) | 0.999 | 0.763 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2.0M Ammonium sulfate 0.1M Sodium HEPES pH 7.0 |