7CEM

Crystal Structure of YbeA CP74 W7F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-10-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	I 4 2 2
Unit cell lengths	128.784, 128.784, 115.964
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.993 - 2.425
R-factor	0.2228
Rwork	0.217
R-free	0.27310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zyo
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.16-3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.520
High resolution limit [Å]	2.425	5.230	2.430
Rmerge	0.142	0.056	0.836
Rmeas	0.148	0.059	0.878
Rpim	0.040	0.016	0.264
Number of reflections	18788	2013	1855
<I/σ(I)>	8.6
Completeness [%]	100.0	100	99.8
Redundancy	13.6	12.8	11
CC(1/2)		0.999	0.763

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	2.0M Ammonium sulfate 0.1M Sodium HEPES pH 7.0