7CEK
Crystal structure of T2R-TTL-BML-284 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-05-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97852 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 105.571, 158.078, 181.292 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.261 - 2.696 |
R-factor | 0.2286 |
Rwork | 0.228 |
R-free | 0.27110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4i55 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.860 |
High resolution limit [Å] | 2.696 | 2.700 |
Number of reflections | 84097 | 13313 |
<I/σ(I)> | 14.53 | |
Completeness [%] | 99.7 | |
Redundancy | 10.9 | |
CC(1/2) | 0.998 | 0.646 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 6% poly(ethylene glycol) 4000, 8% glycerol, 0.1 M MES (pH 6.7), 30 mM CaCl2 and 30 mM MgCl2. |