7CEA

Crystal structure of HUTS-4 Fv-clasp fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 32 2 1
Unit cell lengths	88.367, 88.367, 78.117
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.790 - 2.550
R-factor	0.2527
Rwork	0.252
R-free	0.26590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qq9 4kaq 5xct
RMSD bond length	0.005
RMSD bond angle	0.867
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.183	2.700
High resolution limit [Å]	2.550	2.550
Number of reflections	11865	1860
<I/σ(I)>	17.77
Completeness [%]	99.8
Redundancy	13.5
CC(1/2)	0.999	0.899

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.26 M ammonium sulfate, 0.1 M MES, pH 6.0