7CE5
Methanol-PQQ bound methanol dehydrogenase (MDH) from Methylococcus capsulatus (Bath)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-27 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.900 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 128.612, 211.828, 223.912 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 153.000 - 1.800 |
Rwork | 0.140 |
R-free | 0.17000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4tqo |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 153.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 535098 | 176635 |
<I/σ(I)> | 14.11 | |
Completeness [%] | 99.8 | |
Redundancy | 3.5 | |
CC(1/2) | 0.900 | 0.860 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7.5 | 291.15 | Hepes, PEG600 |