7CE1

Complex STRUCTURE OF TRANSCRIPTION FACTOR SghR with its COGNATE DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9987
Spacegroup name	P 21 21 21
Unit cell lengths	107.900, 218.960, 284.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.149 - 3.200
R-factor	0.2358
Rwork	0.233
R-free	0.28090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7cdv
RMSD bond length	0.009
RMSD bond angle	1.645
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.250
High resolution limit [Å]	3.200	3.200
Number of reflections	987667	111573
<I/σ(I)>	12.9
Completeness [%]	99.8	99.9
Redundancy	5.2	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	10mM Tris-HCl pH 8.5, 100mM NaCl, 1mM EDTA