7CDZ
Crystal structure of 2019-nCoV nucleocapsid N-terminal domain (NTD) protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97919 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.266, 55.430, 85.680 |
| Unit cell angles | 90.00, 95.38, 90.00 |
Refinement procedure
| Resolution | 29.500 - 1.800 |
| R-factor | 0.1987 |
| Rwork | 0.197 |
| R-free | 0.22660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ofz |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.808 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 50503 | 4976 |
| <I/σ(I)> | 32.724 | |
| Completeness [%] | 98.4 | 97.6 |
| Redundancy | 6.6 | 6.6 |
| CC(1/2) | 0.998 | 0.963 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M SPG, pH6.0, 25 % w/v PEG 1500 |
