7CDN
Lysozyme room-temperature structure determined by SS-ROX combined with HAG method, 42 kGy (9000 images)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 298 |
Detector technology | PIXEL |
Collection date | 2019-11-13 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 78.416, 78.416, 38.648 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.450 - 1.700 |
R-factor | 0.1844 |
Rwork | 0.183 |
R-free | 0.21070 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4eta |
RMSD bond length | 0.005 |
RMSD bond angle | 0.750 |
Data reduction software | CrystFEL (0.8.0) |
Data scaling software | CrystFEL (0.7.0) |
Phasing software | PHENIX (1.17.1_3660) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.450 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 13805 | 670 |
<I/σ(I)> | 8.32 | |
Completeness [%] | 100.0 | |
Redundancy | 370.5 | |
CC(1/2) | 0.990 | 0.707 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 293 | The lysozyme solution (40 mg/ml) in 10 mM Na-acetate, pH 4.6 was mixed with the same volume of 4 M Na-Malonate pH 3.1, 6% w/v PEG6000 and stirred for 20 minutes. The crystal growth was stopped by adding 2.4 M Na-Malonate pH 3.1, 3.6 % w/v PEG6000. |