7CDI
Crystal structure of SARS-CoV-2 antibody P2C-1F11 with RBD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-03-30 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9796 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 194.880, 85.390, 58.510 |
Unit cell angles | 90.00, 100.29, 90.00 |
Refinement procedure
Resolution | 33.974 - 2.960 |
R-factor | 0.2046 |
Rwork | 0.202 |
R-free | 0.25380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6m0j |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.15_3459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.980 | 3.040 |
High resolution limit [Å] | 2.960 | 2.960 |
Rmerge | 0.155 | 0.997 |
Number of reflections | 19785 | 1951 |
<I/σ(I)> | 10.1 | |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1M sodium citrate tribasic dihydrate, pH 5.8, 20% PEG 6000 |