7CDI

Crystal structure of SARS-CoV-2 antibody P2C-1F11 with RBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9796
Spacegroup name	C 1 2 1
Unit cell lengths	194.880, 85.390, 58.510
Unit cell angles	90.00, 100.29, 90.00

Refinement procedure

Resolution	33.974 - 2.960
R-factor	0.2046
Rwork	0.202
R-free	0.25380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m0j
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.980	3.040
High resolution limit [Å]	2.960	2.960
Rmerge	0.155	0.997
Number of reflections	19785	1951
<I/σ(I)>	10.1
Completeness [%]	99.7	99.9
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1M sodium citrate tribasic dihydrate, pH 5.8, 20% PEG 6000